Six specific agglutinins were used to identify the terminal sugar residues in the surface oligosaccharides of rabbit and hamster spermatozoa by specific agglutination. Cold agglutinins can cause acrocyanosis and hemolytic anemia. It is a family of similar legume lectins.
Species similarities were found in terminal residues, resembling L-fucose and N-acetylneuraminic acid. When ejaculated rabbit sperm were compared to epididyimal sperm, the latter were more agglutinable with a specific agglutinin recognizing N-acetyl-D-gl. light chains, and only a minority.